March 14, 2013

Determination of Moisture, Total Soluble Solid and Ash Contents



Moisture of products is an important property for food scientists in food industry for several reasons. It has effects on food quality, microbial stability, and food processing operations. Microbial growth is directly related with moisture content because water is vital for microorganisms. Moisture content also is important for achieving quality standards. Moreover knowledge of moisture content of food materials indicates type of processing operations.


Refractometer Scale

Microorganisms need water in an available form to grow in food products. The control of the moisture content in foods is one of the oldest method to prevent from microbial growth. Microorganisms respond differently to moisture content depending on a number of factors. Microbial growth and the production of microbial metabolites may be particularly sensitive to changes in moisture content. Microorganisms generally have optimum and minimum levels of moisture content for growth depending on other growth factors in their environments. Many bacterial pathogens are controlled with moisture contents in the sample. [1]. Moisture content helps determining of microbiological stability of the food materials.

Texture is one of the most important characteristics for the quality of food. Moisture content has a determining effect on the evolution of important food properties, such as texture, color and nutritional value. Controlling the initial moisture content and its migration during storage is critical to the quality and safety of foods. The packaging must provide a good moisture barrier, especially to protect foods from excessive drying out, which adversely affects both texture and taste. [2]
Moisture content affects rheological properties of food product. This situation causes operating different processes and different conditions in production lines such as devices, pipes etc. Moreover there are some limitations and standards those producers have to obey about moisture content. Some operations might be required to achieve.

Total soluble solids content of a solution is determined by the index of refraction. This is measured using a refractometer, and is referred to as the degrees Brix. Brix is the term used when a refractometer equipped with a scale, based on the relationship between refractive indices at 20°C and the percentage by mass of total soluble solids of a pure aqueous sucrose solution. This tests the solids concentration of a sucrose containing solution. It is widely used during fruit and vegetable processing to determine the concentration of sugar in the products. Sugar concentration is expressed in degrees Brix. At 20°C, the Brix is usually considered equivalent to the percentage of sucrose in the solution. The measurement must be made at 20°C to get an accurate value. [3]

Ash is the inorganic residue remaining after water and organic matter have been removed by heating. Total ash content determination in foods provides a measure of total amount of minerals since minerals are not destroyed by heating. The mineral content varies and depends on many factors, such as the variety of wheat, the terrain, the fertilization, and the climate. Moreover, it helps to estimate food’s quality microbial stability and nutrition value.


REFERENCES

[1]Mossel DAA, Corry JEL, Struijk CB, Baird RM. 1995. Essentials of the microbiology of foods: a textbook for advanced studies. Chichester (England): John Wiley and Sons. 699 p.

[2]Weinberg Z.G., Yan Y., Chen Y., Finkelman S., Ashbell G. & Navarro S. 2008. The effect of moisture level on high-moisture maize (Zea mays L.) under hermetic storage conditions-in vitro studies. Journal of Stored Products Research, 44(2), 136-144.

[3] FDA Sercice of Regulatory Offices, (2005, September 10). Food Standards and Additives. ORa Laboratory Manual,  IV-10, 18.

[4] Hanson, J. (2003). Refractometry. Retrieved March 10, 2013 from http://www2.ups.edu/faculty/hanson/labtechniques/refractometry/intro.htm

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